|
KMID : 0616120170480010093
|
|
Korean Journal of Pharmacognosy
2017 Volume.48 No. 1 p.93 ~ p.96
|
|
|
Simultaneous Determination of (+)-Pseudoephedrine and (-)-Ephedrine in Ephedra intermedia by HPLC-UV
|
|
Jeong Bi-Rang
Yoon Yoo-Sik
Shin Soon-Shik
Kwon Yong-Soo
Yang Hee-Jung
|
|
|
Abstract
|
|
|
|
Ephedra alkaloids, (-)-ephedrine, (+)-pseudoephedrine, (-)-N-methylephedrine, (+)-N-methylpseudoephedrine, (-)- norephedrine and (+)-norpseudoephedrine, from ephedra herb are sympathomimetic agonists causing an increase of metabolism, blood pressure and perspiration. In this study, we developed the validation method of (+)-pseudoephedrine and (-)-ephedrine, two major ephedra alkaloids in Ephedra spp., by high-performance liquid chromatography-ultraviolet spectrometer (HPLC-UV). HPLC analysis was performed using a HECTOR-M C18 column operating at 35 ℃, and UV detection at 215nm. The mobile phase used a gradient flow with 25 mM SDS in water (A) and acetonitrile (B).
|
|
|
KEYWORD
|
|
|
HPLC analysis, Ephedra intermedia, Ephedrine, Pseudoephedrine, Method validation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|